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In linearity study, bias for all dilutions was < 20%, and carryover assessment cannot be considered significant on both softwares.

993 for % of CD34+ cells and 0.983 for absolute count of CD34+ cells). Comparison of data showed no statistically significant differences between facscanto clinical and facsdiva software (Spearman's rank correlation coefficients were.

CVs for within-run precision were <10%, except for high absolute count of CD34+ cells on facsdiva software. Resultsįor between-run precision, coefficients of variation (CVs) were all <10%, except for low control level on facsdiva software. Validation study included determination of within-run and between-run precision, trueness (bias), comparison of the test results analyzed on facscanto clinical and facsdiva softwares, assessment of linearity, specimen stability, and carryover. The aim of this study was to evaluate BD Enumeration stem cell kit on flow cytometer BD facscanto II, using facscanto clinical and facsdiva softwares. The modified ISHAGE protocol is the most often used procedure for determination of CD34+ cells using flow cytometry.

CD34+ hematopoietic stem cell (HSC) enumeration by cell flow cytometry is routinely used in clinical laboratories for monitoring of HSC mobilization into peripheral blood and assessment of the quality of HSC products.